Please use this identifier to cite or link to this item:
http://ir.library.ui.edu.ng/handle/123456789/2974
Title: | In-vitro Bactericidal Kinetics of Chlorhexidine Gluconate Disinfectant/ Antiseptic Formulations Containing Different Additives |
Authors: | Idowu, P. A. Idowu, O. S. |
Keywords: | Chlorhexidine gluconate savlon hibiscrub purit bactericidal kinetics |
Issue Date: | Jan-2014 |
Abstract: | Chlorhexidine gluconate(CHG) is a popular disinfectant/antiseptic which is often formulated with additives. We investigated the effect of additives type on the in vitro bactericidal kinetics of CHG in three commercially available formulations: Hibiscrub®, Savlon® and Purit® commonly used as household and hospital disinfectants/antiseptics. The bactericidal kinetics of the products was determined by time-survival curve method using Pseudomonas aeruginosa NCTC 6750 and Staphylococcus aureus NCTC 6571 as model organisms. Best-fit rate constant and half-life was computed by exponential decay curve-fitting. Half-life was; 76.5, 65.5, 66 and 74 (min) for Control solution, Hibiscrub®, Savlon® and Purit® respectively, against Ps. aeruginosa. The corresponding values obtained against Staph. aureus are 51.0, 61.7, 29.3, and 49.0 (min) (95% CI). Presence of alcohol (e.g. Hibiscrub® and Savlon®) caused an insignificant increase in the rate of killing of Ps. aeruginosa relative to preparations that are devoid of alcohol. (p>0.05, 1-way ANOVA). Toward Staphylococcus aureus, the combined effect of cetrimide and alcohol (e.g. Savlon®) is higher than any enhancement due to combination of alcohol and surfactant (e.g. Hibiscrub). Savlon® show a significantly higher bactericidal effect of all the preparations (P<0.0001, 1-way ANOVA). The choice of additives in the formulation of chlorhexidine antiseptic solutions significantly alters the kinetics and overall bactericidal effect of CHG towards Staphylococcus aureus but not Pseudomonas areruginosa. |
URI: | http://ir.library.ui.edu.ng/handle/123456789/2974 |
ISSN: | 1119-5096 |
Appears in Collections: | scholarly works |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
[16]In-vitro Bactericidal Kinetics of Chlorhexidine Gluconate Disinfectant.pdf | 618.16 kB | Adobe PDF | View/Open |
Items in UISpace are protected by copyright, with all rights reserved, unless otherwise indicated.