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dc.contributor.authorDJOUAKA-FOLEFACK, J.R-
dc.date.accessioned2024-04-16T08:42:53Z-
dc.date.available2024-04-16T08:42:53Z-
dc.date.issued2011-
dc.identifier.other119677-
dc.identifier.otherui_thesis_djouaka-folefack j.r_molecular analysis_2011-
dc.identifier.urihttp://ir.library.ui.edu.ng/handle/123456789/8961-
dc.descriptionA thesis in the Department of Zoology submitted to the Faculty of Science in partial fulfilment of the requirements for the degree of DOCTOR OF PHILOSOPHY of the UNIVERITY OF IBADANen_US
dc.description.abstractThe development of resistance to insecticides by Anopheles mosquitoes continues to threaten the success of malaria control programmes in West Africa. Local data on mechanisms and factors causing resistance in the region are scanty. This study was designed to investigate the environmental factors and mechanisms implicated in resistance to pyrethroids by Anopheles gambiae in southwestern Nigeria and southern Benin Republic. Larvae of Anopheles mosquito were collected in 2007 from 19 localities in the six states of southwestern Nigeria and 18 localities in the seven divisions of southern Benin and reared to adults. These were identified morphologically and with Polymerase Chain Reactions (PCR). They were also bioassayed for susceptibility to pyrethroids. Molecular characterisation of pyrethroid resistant phenotypes was carried out using PCR and microarray analyses of the expressed genes. Dissolved Oxygen (DO) and pH were determined using a digital multipurpose meter while physical appearances of breeding sites were assessed visually. Xenobiotic factors such as Spilled Engine Oil (SEO) and agricultural pesticides that might contribute to the emergence of resistance in Anopheles populations were examined through bioassay. Associations between pyrethroid resistance with environmental factors and molecular profiles of Anopheles were evaluated using Chi square. A. gambiae complex genotyped in Nigeria comprised of 73.6 % A. arabiensis and 26.3 % A. gambiae sensu stricto; while those genotyped in Benin were 92.9 % A. gambiae s.s. and 7.0 % A. melas. Pyrethroid resistance in Nigeria and Benin were recorded in 68.4 % and 94.4 % of the localities examined respectively. Breeding sites contaminated with SEO (B-SEO) or Pesticide Residues (B-PR) had low DO (B-SEO = 13.4 ± 1.5 mg/l, B-PR=12.2 ± 1.7 mg/l), the Non-contaminated Breeding sites (B-NC) had higher levels of DO (B-NC=33.1 ± 2.3) and mainly produced pyrethroid-susceptible Anopheles (p<0.05). Significant variations in pH were not recorded. Differences in habitation by resistant-Anopheles in breeding sites contaminated with SEO or pesticide residues were observed. A. gambiae found around the two agricultural sites (Houeyiho, Benin and Ajibode, Nigeria) exposed to synthetic pesticides showed significant levels of pyrethroid resistance with mortality rates of 70.0 % and 89.7% respectively. A. gambiae larvae survived at SEO concentrations below 11.8x10-3 μL/cm2. Ninety six percent of larval mortality resulted from direct cuticle contact with SEO whereas only four percentage mortality was from larval suffocation. A cross resistance phenomenon was recorded with SEO and pyrethroids. A. gambiae showed the presence of elevated frequencies of knock down resistance West (kdr-W) mutations in Benin samples (kdr-W ranged from 0.6 to 0.9) and absence of kdr-W in Nigeria samples. Two detoxification genes (CYP6P3 and CYP6M2) were up-regulated in resistant-Anopheles. Additional detoxification genes specific to agricultural and SEO sites were also over-expressed in the resistant populations. There was an association between residual synthetic pesticides, spilled engine oil and emergence of pyrethroid-resistance in A. gambiae in Nigeria and Benin Republic. The diversified profile of identified metabolic genes reflected the influence of a range of xenobiotics on selection of resistance in mosquitoes.en_US
dc.language.isoenen_US
dc.subjectAnophelesen_US
dc.subjectPyrethroid resistanceen_US
dc.subjectXenobioticsen_US
dc.titleMOLECULAR ANALYSIS OF MECHANISMS AND IDENTIFICATION OF FACTORS OF PYRETHROID RESISTANCE IN ANOPHELES GAMBIAE SENSU LATO IN SOUTHWESTERN NIGERIA AND SOUTHERN BENIN REPUBLICen_US
dc.typeThesisen_US
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