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DC Field | Value | Language |
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dc.contributor.author | Weinder, J. | - |
dc.contributor.author | Cassens, U. | - |
dc.contributor.author | Gohde, W. | - |
dc.contributor.author | Sibrowski, W. | - |
dc.contributor.author | Odaibo, G. | - |
dc.contributor.author | Olaleye, D. | - |
dc.contributor.author | Reichelt, D. | - |
dc.contributor.author | Greve, B. | - |
dc.date.accessioned | 2018-10-16T10:26:20Z | - |
dc.date.available | 2018-10-16T10:26:20Z | - |
dc.date.issued | 2011 | - |
dc.identifier.other | Journal of Virological Methods 172,pp. 22-26 | - |
dc.identifier.uri | http://ir.library.ui.edu.ng/handle/123456789/2496 | - |
dc.description.abstract | Proviral DNAs are being measured increasingly as a marker of the efficacy of highly active anti-retroviral therapy (HAART) and is accepted for the early diagnosis of perinatal HIV-1 infections. This requires a standardized test which enables the detection of a wide range of subtypes worldwide including O, N and circulating recombinant forms (CRFs). Based on a previous publication, a PCR - Test for HIV-1 provirus detection in peripheral blood mononuclear cells (PBMCs) was developed. Blood samples from 80 individuals infected with HIV-1 and 20 persons negative for HIV-1&2 from Africa and Germany were tested for the presence of HIV-1 provirus DNA. The primer system used enables the detection of proviral DNA despite the high concentrations of human DNA. The limit of detection was determined to be 5 copies per 10(5) cells. All 20 samples from persons negative for HIV were negative for HIV-1 proviral DNA while provirus DNA was amplified from 76 of the 80 (95%) samples from persons infected with HIV. The amplified products were detected by gel-electrophoresis, flow cytometry and real-time PCR. All three detection systems provided the same results. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier Ltd | en_US |
dc.title | An improved PCR method for detection of HIV-1 proviral DNA of a wide range of subtypes and recombinant forms circulating globally | en_US |
dc.type | Article | en_US |
Appears in Collections: | scholarly works |
Files in This Item:
File | Description | Size | Format | |
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(48)ui_art_weidner_improved_2011.pdf | 1.44 MB | Adobe PDF | View/Open |
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